Cuvette ChromatographyIntroduction:Chromatography is a method used to separate organic and inorganic compounds so that they can be analyzed. Scientists often use chromatography to determine which basic constituents make up a specific mixture. Chromatography is based on the principle of selective absorption. A botanist named MS Tswett first discovered chromatography in 1906. Tswett used a method called "column chromatography" to separate chlorophylls by pouring a petroleum ether extract of green leaves into a column of calcium carbonate in powder. The compounds slowly descend through the glass column and each chlorophyll compound is absorbed into beds at different heights based on their individual absorption affinity. Forensic scientists currently rely heavily on chromatography to solve crimes. It is also used for various purposes, such as determining the presence of cocaine in urine, alcohol in blood, and the amount of lead in water. Each of these uses is very important and chromatography is the chosen method because it is very reliable and easy to perform. Objective: Our goal was to use paper chromatography to separate various inks (permanent and water-based) into their base pigments. We could use the formula Rf = the distance traveled by each solute (measured to the center of each solute spot) / the distance traveled by the solvent. This would give us the Rf values ​​for each pigment in the ink. These Rf values ​​must be constant for each individual pigment. Apparatus: Chromatography paper Three permanent pens (red, green, blue) Three water-based pens (red, green, blue) Chromatography tank pre-filled with acetone Dryer ˜ Pencil (for various markings)Ø Scissors (used for serration)Ø Ruler (used for all measurements)Ø Bag TiesMethod: The base of the chromatography paper was marked and then cut to form a serrated edge. This edge has been designed to ensure a controlled flow of acetone onto the chromatography paper..