The first obstacle being the actual generation of Lm biofilms. Through extensive literature reviews and assistance, we were able to merge several facets of different articles to develop a protocol producing biofilms. Subsequently, the problem of quantifying biofilm formation plagued my project. I was using Crystal-Violet and colorimetric assays to attempt to quantify biofilms but unfortunately this reagent gave too many errors in our results due to non-differentiation between viable and non-viable cells. I then proposed using a fluorometric test that would further confirm biofilm viability and activity. Using his previous experiences with fluorometric techniques, Dr. Rolf suggested that we use the reagent resazurin. To summarize the protocol, starter cells were incubated overnight and then diluted into microtiter plates where they were allowed to adhere to the well wall at specified time points depending on the sample. The inoculated microtiter plates then underwent a series of washes with PBS buffer to remove free, unattached live cells. cells, followed by air drying and staining with resazurin, finally measurements were taken in 30 minute increments for a total of two hours at the corresponding wavelengths to quantify the activity of resazurin, this was done at 48 hours, 72 hours, 96 hours, different culture times