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Essay / Photosynthesis experiment - 1589
Objective: Experiment #1: The aim of this experiment is to study the effects of baking soda and light intensity on the rate of photosynthesis of green spinach leaves using observing a floating disk.Experiment #2: The purpose of this experiment is to test which colors work best and float under a different light source. Experiment #3: The purpose of this experiment is to test the chromatography of plant pigments using an alcohol test strip. Procedure: Experiment 1: Oxygen Production I added ½ tablespoon of baking soda to 4 cups of water. I added a small drop of liquid soap to the water and swirled to mix. I used the end of a straw and cut out 20 circles of spinach leaves. I pulled the plunger out of the syringe completely and put the leaf circles into the syringe. Then I pushed the piston back. I used the syringe to draw up the baking soda water until the syringe was about ¼ full of liquid. I placed my finger on the tip of the syringe and pulled the plunger as far as possible without removing the plunger. I repeated this step three times. All the leaf circles sank to the bottom of the liquid. I placed the spinach in a clear glass with about 2 inches of baking soda solution. I blocked out all light. I set the lamp with a compact fluorescent bulb. I placed the glass in front of the lamp. I counted the number of circles that floated after each minute for 20 minutes (positive control). I created a negative control by not placing a compact fluorescent bulb and not placing the glass in front of the lamp. I counted the number of circles floating. I repeated the experiment with fresh circles and used plain water and soap for all steps instead of baking soda and soap... middle of paper ...... contains more CO2 as the negative controls. There will be more spinach circles in the red food coloring than in the green and blue food coloring. My hypothesis was confirmed for both experiments. To continue the experiment, I can use a different light source to test the light intensity. For the food coloring experiment, I might use a different food coloring like yellow or orange to test. Experiment 3: The red pigment and green pigment will follow the alcohol higher on the coffee filter than the yellow pigment. There will only be chlorophyll left in the spinach leaf, the yellow leaf will contain chlorophyll and xanthophyll and the red leaf will contain chlorophyll, carotene and xanthophyll. My hypothesis was confirmed. To continue this experiment, I could use frozen chopped spinach leaves, or replace the rubbing alcohol with methyl alcohol..